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KMID : 0545119950050020087
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 2 p.87 ~ p.91
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Molecular Cloning of Acinetobacter sp. WC-17 Gene Encoding Chitinase
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SHIN, WOO CHANG
LEE, DONG SUN/KIM, JONG GUK/HONG, SOON DUCK
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Abstract
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The chitinase gene was cloned from Acinetobacter sp. WC-17 for investigating the genetic control and enzymatic properties of bacterial chitinase. A genomic library of Acinetobacter sp. WC-17 was prepared in E.coli JM109 by using pUC18 as a vector. The chitinase-positive clone containing 3.2kb insert fragment was obtained from 5,000 insert-bearing transformants. The optimum pH and temperature of cloned enzyme were 6.0 and 55℃, respectively. Almost all the chitinase activity of E.coli recombinant was localized in the periplasmic fraction, while most of the enzyme activity of Acinetobacter sp. WC-17 was found in the extracellular fraction.
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